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. Between 2 and 3 M GdnHCl, two partially folded intermediate states possessing flexible tertiary structures (revealed by fluorescence data) but compact secondary structures (as indicated by circular dichroism measurements) were identified
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. Furthermore, studies with solubilizing agents such as detergents, reversed micelles, or nondetergent sulfobetaines (NDSB) proved that aggregation, which typically occurs during thermal unfolding in pure buffers, is strongly suppressed when such agents are added to the solution (29,32)
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- A reporter's e-mail exchange with Carter Center press secretary Deanna Congileo elicits the response that none of the anonymous donors are from the Middle East, but no further details can be released without permission from the donors--which, even if granted, will take some time to obtain (stay tuned)
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. The N [arrow right] I transition, which was referred to as the pretransitional change, ended at ~68°C and the T^sub 1/2^ occurred at ~60°C (see Table 1)
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. Interestingly, the sample with removed GndHCl exhibits now a strong elastic scattering related to pronounced aggregation, which was not observed under unfolding conditions with 8 M GndHCl
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