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. Incoherent inelastic neutron scattering experiments probing the vibrational density of states are scarce but seem to favor an increase of low-frequency, collective motions resulting in increased protein flexibility upon ligand binding (10)
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. To determine the presence of conformational exchange on microsecond and millisecond timescales, the relaxation-compensated CPMG experiment (20) was carried out at 308 K at 500 MHz and at 308 and 300 K at 600 MHz
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. It is tempting to speculate that the CaM dimer opening-closing process is coupled with the CaM:SEF2-1mp interaction, i
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