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Second, the accessibility of the aromatic residues and structural changes occurring upon binding of the effectors to EI^sup SC^ was tested by acrylamide quenching experiments and trypsin digestion experiments account care health savings .
Determination of the rotational correlation timesA reliable estimate of the overall rotation correlation times is very important for the further analysis of the relaxation data account care health savings .
Cross-linking was allowed to proceed for 120 s before quenching by addition of 10 µl of 7% NaBH^sub 4^ in 0 account care health savings .
We treated Gα^sub s^(GDP) with dithiothreitol (see Philip and Scarlata, 2004) and found that its pressure behavior was unchanged (data not shown) account care health savings .
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We followed here a similar procedure to estimate errors on the conformational entropy changes upon complex formation for the sets of atoms that are involved in intermolecular interactions and/or show conformational changes (Table 1) account care health savings .
[FIGURE 1 OMITTED]The oldest eggs of P account care health savings .
To confirm that our labeled ribozyme constructs are active in cleavage, we performed activity assays using a cleavable all-RNA substrate strand, as described in the Materials and Methods section account care health savings .
) McNeill AM, Annest JL account care health savings .
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