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How to small single-domain proteins fold? Fold account cons health pro savings .
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Shearing or compressing a soft glass in 2D: time-concentration superposition account cons health pro savings .
Circular dichroismFar ultraviolet circular dichroism (UV CD) spectra were recorded using a Jobin Yvon (Longjumeau, France) circular dichroism spectropolarimeter in the range 190-260 nm account cons health pro savings .
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Redetermination of the pressure dependence of the lipid bilayer phase transition account cons health pro savings .
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Time-resolved FRET (trFRET) measurements were subsequently used to resolve and quantify the equilibrium distribution of extended and docked ribozyme conformers (N account cons health pro savings .
Hairpin ribozyme activity assayThe substrate strands S2WJ, S3WJ, and S4WJ were labeled radioactively with ^sup 32^P at the 5' terminii account cons health pro savings .
Subsequently, the strands were purified using sephadex G-25 quick spin columns (Roche, Basel, Switzerland) account cons health pro savings .
This issue and appropriate control experiments are discussed further in the Results section account cons health pro savings .
Evidence for rapid interconversion of extended and quasi-docked ribozyme conformationsA notable feature of the SL 4WJ in the presence of high Mg^sup 2+^ concentrations is the markedly asymmetric peak shape in the FRET efficiency histogram, with pronounced tailing on the low-E side of the peak (Fig account cons health pro savings .
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