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. DISCUSSIONMolten globule (MG) folding intermediates have been characterized for a number of proteins (for reviews, see Kuwajima, 1989; Ptitsyn, 1992, 1995)
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. The order of events could be characterized as the intensity decrease of the band at 1629 cm^sup -1^ > the intensity increase of the band at 1617 cm^sup -1^ > the intensity decrease of the band at 1649 and 1658 cm^sup -1^
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. For each of the sequences shown in Table 1
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. Cavity statistics, P^sub oa^(r) folded ubiquitin are shown in Fig
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. Accurate bond and angle parameters for X-ray protein structure refinement
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. Kinetics of aggregation and gelation of globular proteins after heat-induced denaturation
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. The sensitivity of the ANS binding method to the tertiary structure of proteins in ice should find practical utility in devising safer freeze-drying protocols of pharmaceutical proteins and testing the effectiveness of various stabilizing/ destabilizing agents employed in protein formulations
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. John Wiley & Sons, New York
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. 48 g/g) was measured by weighing shortly before closing the sample holder
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. It is noteworthy that all G-protein subunits are posttranslationally modified with hydrocarbon chains (see Wedegaertner et al
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.Biophysical Characterization of the Enzyme I of the Streptomyces coelicolor Phosphoenolpyruvate:Sugar Phosphotransferase SystemHurtado-Gómez, EstefaníaABSTRACT The first protein in the bacterial phosphoenolpyruvate (PEP):sugar phosphotransferase system is the homodimeric 60-kDa enzyme I (EI), which autophosphorylates in the presence of PEP and Mg^sup 2+^
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. Characterization of cold denaturation and urea-protein interactions
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. Here, we compare the ANS binding capacity of the four azurin species to inquire how these modified features modulate the ice-induced perturbation of tertiary structure
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. In other words, the second MD trajectory segment covers a small subspace of the conformational space sampled by the first segment and hence reduces the overall size of the elements of the covariance matrix σ through averaging over the combined trajectories
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