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. It is noteworthy that the same results could be obtained for the diluted sample with a concentration (0
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. For the longest sequence in our data set-the sequence of villin-for which N = 126, generation of the desired conformational ensemble takes ~ 20 h on a single 2
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. 2, N is the number of residues, and R^sub ij^ is the distance between atoms i and j
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. The peak maxima range from ~308 nm for relatively nonpolar environments inside the protein globule to λ^sub max^-values of 350 nm which represent chromophores at the protein surface surrounded by polar water molecules (5,6)
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. However, flexible polymers and lower molecular weight components typically vitrify at much lower water contents (We thank Dr
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. For the high-resolution imaging, the AFM head (with optical block and base) was placed atop a commercially available active (piezo-actuated) vibrations-damping desk (from Halcyonics, Göttingen, Germany)
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. The herein-reported multiple pathways of insulin amyloidogenesis and the resulting heterogeneity of the mature amyloid illustrate the kinetically controlled character of the process
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. Consider the short-chain fatty acids (SCFAs), one of which (butyric acid) is found almost exclusively in milk fat
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. Our results highlight the varying contributions of preformed secondary structure elements to tertiary folding of the hairpin ribozyme
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. Docked fractions calculated from relative areas of the peaks are plotted in Fig
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. Van GerreyA: Well, I suppose it would be possible, but I fear I must rain on your picnic today
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. 5 ml papain digest buffer (Sodium citrate (55 mM), Sodium chloride (150 mM), Cysteine Hydrochloride (5 mM), EDTA (5 mM) (all Merck, Poole, UK)); and 1 µg/ml papain (Sigma, Poole, UK)
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. In vitro chondrogenesis of bone marrow-derived mesenchymal progenitor cells
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. 99--among other items, under the heading "New Year, New You
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. DiscussionLong-term probabilities by themselves are not sufficient explanations of risk-taking behavior
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. A number of MD simulations have been carried out to shed light on this topic
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. Secreted DmAChE was purified to homogeneity using the following steps: 70% ammonium sulfate precipitation, dialysis, affinity chromatographies on a procainamide-Sepharose column and on nitrilotriacetic acid-nickel agarose (39)
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. The distance between DNA helixes in a compacted DNA can largely vary as a function of PEG concentration from 25 [Angstrom] to 38 [Angstrom] (Maniatis et al
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. 5, C and D) and shorter fluorescence lifetimes (data not shown) reveal Trp to ANS and Bis-ANS fluorescence resonance energy transfer both in the native and misfolded states, indicating that the Tips and the probes are in close proximity (Conrad and Brand, 1968)
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. The microscope was coupled to an AS-12 E-scanner (13-µm) and an Extender Electronics Module EX-II (Digital Instruments, Santa Barbara, CA), which allows acquisition of phase images
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. Underlined items in the "Abundance" column indicatethat some or all of the specimens were preserved and at least oneindividual was correctly identified as Pseudopercis semifasciata
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. Holding the solution at 130°C for 2 hours and subsequently at 160°C for 2 hours produced an average particle size (51 µm) comparable to that (58 µm) obtained by holding at 160°C for 2 hours and then for 2 hours at 130°C
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. We are especially grateful for all those unpaid volunteers who helped with fieldwork: Joe O'Malley, Mark Amend, Bill Pinnix, Wolfe Wagman, and Pat McDonald
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.Red sea bream (Pagrus major) (Table 1) eggs can easily be distinguished from those of other sparids also found in Japan, such as Acanthopagrus latus, by differences in egg size and spawning seasons, and from those of Evynnis japonica by differences in spawning seasons (Ikeda and Mito, 1988; Kinoshita, 1988; Hayashi, 2000)
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.) Yet after Wafer's hearing, nearly 30 more people, some in cases overseen by the same judge, were convicted of selling Coleman cocaine based on his say-so or pleaded guilty after seeing the stiff sentences that were coming down
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