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. The N [arrow right] I transition, which was referred to as the pretransitional change, ended at ~68°C and the T^sub 1/2^ occurred at ~60°C (see Table 1)
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. A trace from a fluorescence anisotropy decay measurement of aggregates (incubated under A-state conditions for 24 h) is shown in Fig
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. There are four families of Gα-subunits, and phospholipase C-β(PLCβ) is the main effector of the Gα^sub q^ family (Rebecchi and Pentyala, 2000; Rhee, 2001)
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. METHODSThree simulations of 10 ns at 300 K, 1 atm, were carried out for three different systems in explicit solvent: free HIV-1 gp120 with the truncated LAI strain sequence (SWISS-PROT accession number P03377; 346 residues and 29,047 water molecules), free CD4 (99 residues of the D1 domain and 7768 water molecules), and their complex (445 residues and 30,242 water molecules)
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