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The Mn^sup 2+^ and Ca^sup 2+^ concentrations used were 5 mM in all the experiments unless specified otherwise affordable agency family health insurance .
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In essence, the structure consisted of an α-helix subdomain, which is a four-helix bundle, comprising α-helices 1-4; an α/β subdomain, comprising a β-sandwich, formed by a four-stranded parallel β-sheet (β-strands 1-4) and a three-stranded antiparallel β-sheet (β-strands 1, 5, and 6), and three short helices (α-helices 5-7) that contain the phosphorylation site (His^sup 186^) (Fig affordable agency family health insurance .
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Therefore, the maximum of the fluorescence emission spectrum (λ^sub max^) is sensitive for the polarity of the microenvironment of the intrinsic fluorophore affordable agency family health insurance .
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This feature is consistent with interconversion between compact and extended structures, which we estimate takes place on the 100-µs timescale using a simple model for the peak shape affordable agency family health insurance .
Role of secondary structure elements in tertiary foldingThe systematic study of hairpin ribozyme constructs containing different combinations of helical junction and loop elements presented here enables us to define the role of these secondary structural elements during tertiary folding affordable agency family health insurance .
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