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. A note on the molar absorptivity of reduced Ellman's reagent, 3-carboxylato-4-nitrothiophenolate
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. The hydrodynamic radii (R^sub h^) for denatured proteins scale with chain length (N) as: R^sub h^ = 2
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. The triglyceride peaks were better resolved, and small additional peaks-creatine, choline, and phosphatidylcholine at 2
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. The linewidth of the diminazene peak in the nucleus is initially about twice as broad as that of aceturate and approaches the latter over time, whereas the linewidths of both molecules in the medium are nearly identical
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. Structural transitions of deoxyribonucleic acid in aqueous electrolyte solutions
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. Formation of an amyloid reflects an alternative to the native packing conformational struggle of a polypeptide chain to 1), reduce its surface accessible area; 2), saturate hydrogen bonding; and 3), reach an alternative non-native global free energy minimum (Gazit, 2002)
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. In contrast, the specifically-docked conformation (exemplified by the WT 4WJ) is stabilized by an extensive hydrogen bond network (14 hydrogen bonds in all (Rupert and Ferre-D'Amare, 2001)), prolonging the lifetime of this state
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. Reaction was started by addition of 1
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. Implications for reconstitution of the active enzyme
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. Similarly, if the genes that control eye degeneration in cave fish also control other, unrelated structures that could confer an advantage to fish living in darkness, pleiotropy could explain the loss of eyes
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. Consistent with previous observations (26), the intensity of the intrinsic Trp fluorescence decreased with the increase of temperature, and meanwhile, the emission maximum wavelength red shifted from ~333 nm to 340 nm (Fig
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. Although the glycosylated form undergoes a simple two-state unfolding, the nonglycosylated species unfolds via a compact monomeric intermediate that is not a molten globule
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. Our presentation is organized as follows
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. Peptides do not contain information that goes beyond local propensities and these do not provide insights regarding the correlated fluctuations required to explain how scale invariance of structural, colligative, and thermodynamic properties come about
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. the enzyme was more resistant to proteolysis (open and solid circles) (Fig
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. The fact that for NDSB samples the obtained Δλ^sub max^-value between the folded and the unfolded state is much larger as compared to that measured with pure buffer samples is a further indication that aggregates reduce the magnitude of red shifts
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. John Wiley & Sons, Chichester
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. MSDs of native and soman-inhibited HuBChE were identical below the thermal denaturation temperature of the native enzyme, indicating a common mean free-energy surface
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. The RT^sup n^^sub 1^ value is always 1 in the absence of internal motion or when the internal motion is faster than ~200 ps
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., 2004), and insulin (Bouchard et al
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. Small particles were suspended in the solvent using the particle size analyzer, then an extraction process [10] was run with acetone and methanol (Figure 3)
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.Notes: The bold values indicate period-specificaverage monthly rates relative to rates in thepre-RP period
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