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Predissociated Dimers and Molten Globule Monomers in the Equilibrium Unfolding of Yeast Glutathione ReductaseLouzada, Paulo RobertoABSTRACTThe equilibrium unfolding of dimeric yeast glutathione reductase (GR) by guanidine hydrochloride (GdnHCl) was investigated affordable group health illinois in insurance .
5 M GdnHCl caused only a minor decrease in ellipticity at 222 nm (Fig affordable group health illinois in insurance .
This behavior is expected to hold for all |i - j| > n^sub s^, where n^sub s^ denotes the number of residues over which sequence context is important affordable group health illinois in insurance .
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The rotational correlation time was estimated in the range 13-16 ns affordable group health illinois in insurance .
If this idea is correct, then the volume associated with Gβγ activation of PLCβ corresponds to the difference in volume changes for PLCβ-Gβγ alone and in the presence of Gα^sub s^(GDP), ranging from ΔV ~3 to 15 ml/mol (Table 1) affordable group health illinois in insurance .
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