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. Investigation of the properties of these partially folded states using probes such as ANS or bis-ANS (which bind to exposed hydrophobic domains in proteins) was not possible due to the significant overlap between the emission spectra of these probes when bound to proteins and the excitation spectrum of the prosthetic group, FAD (data not shown)
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. of Biomedical Engineering and Center for Computational Biology, Washington University in St
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. 5; monosodium dihydrogen phosphate, pH 6
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. We attempted, by docking of the EIN^sup sc^ and EIC^sup sc^ domains, to model the whole structure of EI^sup sc^ trying to keep the active-site histidine (in the EIN^sup sc^ domain) close to the PEP binding site (in the EIC^sup sc^ domain)
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. With respect to all α-amylases investigated here, TAKA is the only protein which does not show a visible aggregation upon thermal unfolding (17)
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. Therefore, correlating the wetting balance tests results and the morphological analysis, it can be concluded that the higher soldering temperature enhances the wettability due to the rapid chemical reactions between the solder and the substrate
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. They generally have k^sup -1^^sub ex^ values below 100 µs confirming the trend
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. 4 or in water-methanol mixtures at pH 7
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. The fitted rotational correlation time for native TTR using bound ANS was found to be 27 ns, and for native TTR with bound Bis-ANS 22 ns
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. Gestation period is likely to vary with water temperature
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