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. Aminoacylase activity assay The enzyme was dissolved in 30 mM Tris-HCl, pH 7
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. Modern polymer theories have established that the use of Flory's mean-field model is flawed when it comes to predicting detailed properties of conformational ensembles in the EV limit (38,39,41,42)
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. If one were to examine chain properties at length scales that go above l^sub s^, properties of denatured proteins for different sequences should become indistinguishable from each other
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. Pervasive conformational fluctuations on microsecond time scales in fibronectin type III domain
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.9, or increased above 10, the presence of a second slower diffusive process in the autocorrelation function was observed, which was attributed to a very limited aggregation/association of BLG (34)
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. After the buffer baseline subtraction, the data were normalized to protein concentration and expressed as molar residue duplicity, [Θ], No changes in [Θ] were detected with changes in the protein concentration in the range explored (5-600 µg/ml)
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., 1981), lipid binding increases the helix content in apoA-2 to ~64%
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