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. The native ACYI contained Class I (~60%) and II (~40%) fluorophores, but did not contain Classes A, S, or III (Fig
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. It is also noteworthy that the observed scaling behavior is valid for a range of chain lengths that includes short chains (11)
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. The large-scale motion generated by backbone pivot moves ensures a lack of correlation between saved snapshots
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. RESULTS AND DISCUSSIONCharacterization of soluble prefibrillar aggregates and protofibrilsGeneration of prefibrillar oligomers of TTR from the A-stateProlonged dialysis of TTR (96 h, 4°C) versus 10 m M HCl in dH^sub 2^O in the absence of salt (pH 2
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. ANS binds to a small extent to the unfolded monomeric form of TTR (Table 2), providing an excellent reference point for molecular dynamics studies of the quaternary structure of TTR
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. In this work, we set n = 14
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. In conclusion, from examining azurin mutants of lower thermodynamic stability and greater internal flexibility than the WT, it was found that the perturbation of the native fold in the frozen state, evidenced by the capacity to bind ANS, is enhanced by the plasticity of the globular structure and correlates with the lability to freeze-thawing
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.2, but also indicates that contributions to larger values (including the nonclassical range Φ > 1) may arise from nonnative interactions with that residue that serve to restrict the folding space
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.5 ns, resulting in a final increase of 0
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.Researchers have reported on polymer particle formation by phase separation [2, 9, 12, 16], but these works have not included the use and testing of the particles as pigment-loaded toner for xerography
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. Ribozyme constructs missing the substrate strand were prepared in annealing buffer (missing NaCl and supplemented with 12 mM MgCl^sub 2^) at a concentration of 2 µM of each strand and annealed by heating to 70°C for 2 min and cooling to ambient temperature
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