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Claudia Rosett is a journalist-in-residence with the Foundation for Defense of Democracies
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. Ali argued such moves could lead to further development andprosperity in Asia
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. Taken together, these results suggest the existence of two molten-globule-like intermediate species (one dimeric and one monomeric) in the unfolding of GR
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. Native dimeric GR eluted at a volume corresponding to an apparent molecular weight of ~85 kDa in the calibrated column, indicating a fairly compact dimer structure
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. The R^sub g^ of molten globule (MG) state is close to that of N state rather than U^sub A^ state
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. The precipitate was resuspended m 6 M guanidinium hydrochloride (in 10 mM Tris buffer (pH 7
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. A summary of our results from analysis of EV-limit ensembles for 23 different two-state proteins is provided below: 1
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. SWISS- MODEL: an automated protein homology-modelling server
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. 20 mg/ml unfolded TTR at pH 2
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. This was used as a reference as well as to extract novel information about the properties of DCVJ and ThT through this comparison (more below)
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. Fluorescence anisotropy data from Bis-ANS bound to the different unfolded and aggregated states showed modest anisotropy values (compared to when bound to the thyroxine binding site in the native TTR tetramer), revealing that Bis-ANS docs not penetrate the protein but is more loosely associated to the protein surface (data not shown)
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. The objective of our research presented in the present article is twofold
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. However, those authors propose that this phenomenon is unlikely to have physiological relevance, since the inactivation of GR is very slow at cellular NADPH concentrations
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. Thermal unfolding transitions were monitored by taking the CD signal at 222 nm as a function of temperature
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. As a final remark we point out the close association found between ANS binding capacity (relative area, Table 2) and the peak wavelength of the fluorescence spectrum, λ^sub max^, the latter shifting further to the blue as the binding capacity increases
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. The inverse relationship between protein dynamics and stability
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. A relaxation-compensated Carr-Purcell-Meiboom-Gill sequence for characterizing chemical exchange by NMR spectroscopy
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. Three probes-ANS, DCVJ, and ThT-show increased fluorescence throughout the aggregation reaction, whereas Bis-ANS shows fluorescence decay (Fig
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. The plateau level is preserved for DCVJ over 300 h, showing that this probe also binds to larger assemblies (spherical aggregates and protofibrils)
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. Linked folding and anion binding of the bacillus subtilis ribonuclease P protein
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. However, because we have compared results across a series of molecules where the RNA sequence (and secondary structure) in the vicinity of the dyes has been held constant, the differences between the characteristics we observe are related primarily to tertiary structural differences between the constructs
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