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Although the thermal unfolding of many proteins can successfully be illustrated by a two-state model, step-wise transitions have been observed in several proteins regardless of whether the process was reversible or irreversible (30,38, 39,49-54)
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That the intermediate is monomeric in nature is also evident from its fluorescence emission λ^sub max^, which is similar to its fully folded monomeric state, characterized earlier (23) (Fig
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By a combined effort of thermal and chaotrope-induced denaturation we have been able to show the profound effect of glycosylation in the confomiational stability and oligomerization of the protein
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(46) used pulse-field gradient NMR to quantify effective hydrodynamic radii for seven denatured proteins, the lengths of which varied from 16 to 247 residues
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The ability to simulate denatured-state ensembles is important for a range of applications including protein design (25,26)
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The large fitting errors are probably due to the steepness of the experimental data between pH 5 and 6
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1 in the concentration range 45-54% w/w was determined by oscillatory rheometry
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We found no changes in DNS fluorescence over 1-2000 bars, suggesting stable membrane binding even through the phase transition (Fig
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DISCUSSIONIn this study, we have applied high pressure techniques to determine the volume change associated with PLCβ-Gβγ interactions on membrane surfaces and in buffer
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Universidad National del Sur, Bahia Blanca, Buenos Aires, Argentina
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Although SCFAs are saturated, they are not associated with elevated blood cholesterol and, thus, could be considered to be misrepresented in our nutritional labeling system, in that saturated fats are defined by chemistry rather than by physiological function
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Homogeneous nucleation takes place at or near the surface of the globules, and growth proceeds along the globule walls, giving rise to smooth particles
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Of the 39 cases of mesothelioma, 12 were tubulopapillary and 27 were composed of solid sheets and nests of mesothelioma cells
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Food habits of the Great Horned Owl (Bubo virginianus) in the Cape Region of Eower California, Mexico
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The samples were analysed using a microplate fluorimeter (Fluoroskan Ascent, LabSystems, Oxford, UK), with excitation set at 360 nm and emission monitored at 460 nm
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Facing a system fraught with its own afflictions, the wait may be a long one
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The agency credited increased criminal investigations, "heightened vigilance and awareness by all parties in the drug distribution system" and other factors for the gains
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After being detained on several more occasions, mostly while traveling in Europe, I realized my Blackness and femininity had become my burden
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To date, many national and regional drug store chains, state pharmacy associations, pharmacy schools and independent pharmacists around the country have received AstraZeneca materials
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An increasing number of major employers like General Motors, IBM and DuPont also have adopted mandatory mail
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"I knew then I wanted to be rewarded for my intelligence, as well as my physical labor," he says
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