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Infrared spectroscopy The IR samples were prepared by dissolving ACYI in 100 mM deuterated phosphate saline buffer at a concentration of 50 mg/ml
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FTIR spectra in the presence of the effectors were acquired and compared with those obtained in their absence to find out whether there were secondary structural changes upon binding
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J Immunol 2001; 166:6847-6854(35) Cowan KN, Leung WC, Mar C, et al
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The final water content was measured by weighing shortly before closing the sample holders and was 0
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However, the present data cannot exclude internal motion involving reorientation of the CaM monomers, which have an excepted timescale of ~7-9 ns
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In addition ANS shows a minor slow second phase in response to formation of larger assemblies (spherical aggregates and protofibrils)
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The effect of high pressure on the membrane association of different types of peripherally bound and integral membrane proteins have been reported, and their pressure behavior is variable (e
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It provides a suitable structural template for molecular dynamics (MD) studies allowing a significant reduction in the size for the simulation system as compared to full-length gp120
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He adds that the yellow-green regions of CG4 reveal red-absorbing dust particles that lie between the cloud and Earth
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At 5 wt%, we observed that a continuous gel was not formed, as we had found with the higher polymer concentrations
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05It's the ultimate precision trimmer for slender eyeliners and oversized crayons
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For pharmaceutical companies, Abramson's behavior meant new customers
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than less expensive generic alternatives," Kravitz says
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In a more recent survey of 643 U
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One of five prescriptions includes at least one payment error
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We also consulted with several national and regional drag store chains
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In the hours after Katrina spawned massive flooding in New Orleans and other Gulf State regions, pharmacy leaders from CVS, Walgreens, Wal-Mart, H-E-B, Rite Aid, Kroger Co
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"On the pharmacy side, it became very clear that people were going to be sent to different places" where medical attention was being delivered
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The move to third party plans, said Dan Haron, senior vice president of pharmacy for Brooks-Eckerd Pharmacy, "has been the biggest thing that's happened in pharmacy
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These results further indicated that type of motivation can lead to various levels of participation
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3 billion by 2009, according to a new report from Juniper Research
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"[P]oker is now our most popular card game--maybe our favorite game, period
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That's about 10 million lonely hearts -- good odds for any gambler
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If you take a closer look, though, you can distinguish at least seven habitats
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Other animals on the ridge are the eastern indigo snake (which can grow to more than eight feet long, making it the longest non venomous snake species in North America), the Florida black bear, the Florida gopher frog, the Florida mouse, the Florida pine snake, the Florida sandhill crane, the Florida scrub lizard, the gopher tortoise, Sherman's fox squirrel, and the short-tailed snake
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.After shopping around, Stone, a management consultant for NeighborWorks America, met Leighann Sudhoff, a representative of CFIC Home Mortgage
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. R^sub e^, and internal distances scale with sequence separation according to universal power laws
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. Analysis of the pH- and thermal-denaturation curves The fittings to Eqs
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. In EI^sup sc^, a single peak was observed in CD and DSC experiments, but in the pH range where the thermal transitions could be followed (pH 7-9), two different peaks were not observed; further, the T^sub m^ of the sole transition observed was pH-indcpendent between pH 7 and 9 (Fig
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. The aromatic chromophore 1-anilino-8-naphthalene sulfonate (ANS) is feebly fluorescent in water, but its spectrum is blue shifted and its intensity is dramatically increased in nonpolar solvents or when it binds to nonpolar sites of proteins (4,5; for a general review, see Slavik (6))
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. This suggests that perturbations of the tertiary structure are widespread and implies that the frozen medium is a harsh environment for many globular folds
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. We conclude that yolk platelets-lipoprotein assemblies of different sizes (34)-have caused the inhomogeneities in the vegetal cytoplasm, perhaps along with mitochondria and other organelles with paramagnetic components
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.13 Therefore, only very thin tarnish layers can be removed from the substrate surfaces and result in poor wetting
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. On the other hand, the poor cleaning capability and higher solder-flux interfacial tension (445 mN/mm) of R-type flux are responsible for its worst performances
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. However, newly developed Sn-2
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. The reversible transformation of β-lactoglobulin at pH 7
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. The microscope was coupled to an AS-12 E-scanner (13-µm) and an Extender Electronics Module EX-II (Digital Instruments, Santa Barbara, CA), which allows acquisition of phase images
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.[FIGURE 11 OMITTED]DiscussionOvarian development for black rockfish in Oregon was similar to the developmental cycles reported for other rockfish species (Moser, 1967; Bowers, 1992; Nichol and Pikitch, 1994) with the exception of seasonal timing and stage duration
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. For each of these identified points, the constituent 50-µs binned data was then used to calculate the "burst duration" during this time bin, by determining the total time spent above a threshold of 4 counts
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.12 Thus far, immunohistochemistry has played only an adjunct, minor role in differentiating mesothelioma from reactive mesothelial proliferations
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. The medium was changed every two days and pellet cultures were removed at days 2, 7 and 14 for subsequent analysis
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.The prejudices such decisions reflect are more pharmacological than racial
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