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. However, with respect to the heating rate dependence of the respective λ^sub max^-values obtained for unfolded states, TAKA exhibits the same behavior as compared to the other α-amylases (Fig
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. Several general predictions result: 1), intermediate subspaces of configurations will always be visited; 2), specific but nonnative interactions may be important in stabilizing these low-dimensional diffusive searches on the folding pathway, as well as native interactions; 3), sequential barriers will commonly be found, even in "two-state" proteins; 4), very early times will show characteristic departures from single-exponential kinetics; and 5), contributions of nonnative interactions to Φ-values and "Chevron plots" are calculable, and may be significant
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