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. For each of the sequences shown in Table 1
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. Predicting the energetics of osmolyte-induced protein folding/unfolding
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. The structure of EI^sup SC^ was obtained by homology modeling of the isolated N- and C-terminal domains of other EI proteins
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. Converse to observations in other EI proteins by DSC (6) and CD (52)
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. For this reason, the IMC layer thickness was found from Fig
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. 5 the protein conformation undergoes a reversible transition which leads to a swelling of the monomeric unit (29,30)
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. The V4 loop (residues 398-409), for which electron density is missing in all crystal structures, and the V3 loop, which has been proposed to undergo significant rearrangements upon CD4 binding, were modeled onto the gp120 core, together with the six core mutations of the LAI strain (98
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. The wave numbers used in the calculations range from q = 0 to q = 0
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. At the other end of the spectrum, recent experimental work and some modeling efforts suggest that a new paradigm is in order for denatured-state ensembles (100,101,130)
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.Mutation studies on E12 (a bHLH protein similar to SEF2-1) have shown a gradual decrease in CaM affinity upon each mutation of an interacting residue, and not the on-off behavior usually seen with lock-and-key or induced-fit binding mechanisms (15)
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