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. The gyration tensor is computed as an outer product of the radius of gyration vector
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. Firstly, those residues making van der Waals clashes were selected and lilted with "Quick and Dirty" algorithms: and secondly, models were energy minimized (100 steps of steepest descent and 100 conjugate gradients, with a cut-off of 10 [Angstrom] for nonhonded interactions) with Insight II (Biosym/MSI)
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. 2 M^sup -1^ and v = 0
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. Changes in the CD signal at 208 nm (where the parallel polarized π-π* transition occurs) are the result of a flexibility of the structure (46)
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. Details about site-directed mutagenesis, protein expression, isolation, and purification of mutants I7S, F110S, and C3A/ C26A have been described elsewhere (22,24)
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. 1 are all in nonhelical regions except residues 72, 73, and 75, which are pail of the less well-defined C-terminal part of helix IV
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. The shortest incubation at 60°C (or 70°C) applied to insulin samples was only 30 s; yet as Fig
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- * In Seattle, the terra-cotta domed roof of First United Methodist Church will soon make way for a highrise office tower
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. The final concentrations were 5 mM MgCl^sub 2^ and 2
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