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. The fluorescence spectra were normalized before calculation
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. The question we ask is, what is the probability that a sphere of radius a placed at random with respect to the center of mass of the chain will be empty? For each conformation in the EV-limit ensemble, we place a probe sphere of radius a at several random locations with respect to the center of mass and quantity the number of times a chain atom crosses the probe sphere
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. These observations provide the necessary impetus for developing an accurate statistical thermodynamics framework for understanding how polypeptides respond to increasing concentrations of denaturants to yield ensembles that converge upon the EV limit description
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. Our efforts to develop an accurate EV limit description for the denatured-state ensemble parallels the efforts of the Sosnick (124) and Zhou groups (114)
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. Standard suppliers were used for all other chemicals
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. Second, the accessibility of the aromatic residues and structural changes occurring upon binding of the effectors to EI^sup SC^ was tested by acrylamide quenching experiments and trypsin digestion experiments
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. Spectroscopic imaging and gradient-echo microscopy on a single cell
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. Condensed phases of DNA: structures and phase transitions
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. Gα^sub s^ is modified by two palmitoyl chains (Kleuss and Krause, 2003) which are thought to contribute to its membrane stability
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. In addition, CD spectra of DMPC-bound apoA-2 were recorded from solutions of 5-600 µg/ml protein concentration placed in 10-0
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. Relaxation functions in folding kineticsWe first take a very simple case: if the nonnative gutter-stabilizing interactions are perfectly balanced with the entropy changes at each stage of the dimension reduction, then the free-energy profile is itself flat, and the d diffusive dimensions form an effective one-dimensional path along which the folding takes place
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. Evidence for sequential barriers and obligatory intermediates in apparent two-state protein folding
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. 48), and the anus was situated near the midpoint of the body (Fig
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. , Dodson and Wexlar 1979, Duke and Rhoades 1977, Duke et al
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. Lesson Plans for Student ActivitiesPREPARATION: Before beginning the lessons, make these photocopies: two copies for each student of Reproducible 1 for a pre-reading and post-reading quiz, and one copy for each student of Reproducible 2
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. We also wanted to try to help pharmacists better understand and communicate information about the new Medicare Prescription Drug Benefit to facilitate conversations with beneficiaries and to help them reach out in the community
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- ''China reformed its exchange system in July last year and raisedthe value of the yuan, abandoning its dollar-peg regime and linkingthe yuan to a basket of foreign currencies
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. Details with regard to the intrinsic fluorescence experiments were the same as those described before (30)
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. This is mainly because of the short spatial range of EV interactions
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. Our results show that dimeric EI^sup sc^ acquired a folded conformation at physiological pH: however, 8-anilino-1-naphthalene-sulfonic acid (ANS)-binding experiments, FTIR, and CD measurements suggested that the protein populated a molten-globule-like species at low pH
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. The dimerization of insulin
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. These substrates were then coated by flux and used for testing
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. Aliquots of the aggregation reaction was withdrawn and assayed to avoid adverse effects of probe binding during the reaction
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. Interpreting the folding kinetics of helical proteins
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.0) n=20Table 3Body proportions (mean [[+ or -]SE] and range) of Pseudopercissemifasciata posttransition juveniles
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."There's a wide range of cyclosporins, and the ASP on that seems to go with the lowest-cost drug in that category," she complained
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. Journal of Social Psychology, 142, 405-424
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