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. Interestingly, no significant spectral changes were detected up to 1 M GdnHCl, whereas an increase in reactivity of one thiol group of GR to DTNB was observed at this concentration of GdnHCl (Fig
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. The co-founder of the theory of evolution by natural selection gave an explanation that was, in the end, remarkably Lamarckian
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. How random is a highly denatured protein
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. Three-point calibration of lhe pH-meler was performed by using slandards from Radiometer
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. The model was tested in terms of energy with Fold-X (http://foldx
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. In addition, using the simple general equation, which covers for both fast and slow exchange, derived by Ishima and Torchia (34), we also show that the exchange rate can be determined from a combination of CPMG measured R^sub ex^ and change in R^sub ex^, (ΔR^sub ex^, vide infra) without prior assumption on the exchange timescale
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. The fluorescent dyes 1-anilinonaphthalene-8-sulfonate (ANS), 4-4-bis-1-phenylamino-8-naphthalene sulfonate (Bis-ANS), 4-(dicyanovinyl)-julolidine (DCVJ), and thioflavin T (ThT) were employed in both static and kinetic assays to characterize these oligomeric and protofibrillar states using both steady-state and time-resolved fluorescence techniques
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. The magnetite crystals are believed to have formed from the thermal decomposition of the siderite crystals in the reaction 3FeCO^sub 3^ -[arrow right] Fe^sub 3^O^sub 4^ + 2CO^sub 2^ + CO
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. MATERIALS AND METHODSRNA constructs2'-0-bis(2-acetoxyethoxy)methyl orthoester (2'-ACE) protected RNA strands were purchased from Dharmacon (Boulder, CO)
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