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. "We're not a church that is about to go bankrupt," said the church's pastor, Andrew Mullins
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. "The denials are another way of controlling women's reproductive-health choices," says Faye Wattleton, president of the Center for the Advancement of Women in New York City and former president of the Planned Parenthood Federation of America
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. Also understand that in most states it's not illegal for a school to require a student to take medication in order to attend; many schools may even say that they are "not equipped" to teach certain children
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. As a zinc-dependent enzyme, ACYI is quite stable against heat-induced denaturation and can be regarded as a thermostable enzyme with an optimal temperature for activity of ~65°C
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. Experiments that only detect close spatial contacts will be interpreted as providing evidence of long-range "residual" structure in the denatured state (94-98)
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. 13 shows a comparison of P(x) predicted by theory to distributions computed for four different proteins in the EV limit
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. Taken together, these data suggest that there were structural changes, which partially solvent-exposed the indole moiety and/or tyrosine residues, and modified the ellipticity at 222 nm (41,42)
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. Differential effects of apoptotic versus lysed cells on macrophage production of cytokines: role of proteases
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. NMR Spectroscopy of single neurons
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. The effect is due to the limitation of the instrumental resolution regarding the measurement of hydration water large-scale motions (40)
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. Stefan Hermann's present address is Dept
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. The fluorescence intensity of the different probes in the presence of the unfolded monomer state is shown in Fig
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. To determine whether this could be the case for PLCβ^sub 2^, we monitored the change in emission of CPM-PLCβ^sub 2^ under pressure
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. , neurotoxic protofibrils of α-synuclein-Rochet et al
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. We remark that in both these cases, the stabilized hypergutter provides an arena in which "diffusion-collision" calculations can operate within a molten globule, so constituting a significant generalization of that model to nonspatial degrees of freedom (Zhou and Karplus, 1999)
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. As in the case of the Φ-value analysis, this experiment is also usually interpreted as giving information about the transition state (Oliveberg et al
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. DISCUSSIONSufficient conformational sampling is a prerequisite for a reliable estimation of the conformational entropy
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. In addition, we observed a significant decrease in the proportion of mature fish age 10 or older over the three years of our study (Pearson's [chi square]=52
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. "It's going to happen," he insists, "and it's actually going to end up costing a lot more than Katrina did
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. In this research, the sequential events in ACYI thermal denaturation were investigated by a combination of spectroscopic methods and related resolution-enhancing techniques
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. Native-like mean structure in the unfolded ensemble of small proteins
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. A stressed condition of the native structure in ice appears to be common to most proteins even if oligomers seem to be more susceptible than monomers presumably because the globular fold can be further destabilized by subunit dissociation
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. The CaM:SEF2-1mp complex is found to be highly flexible with internal motions on the picosecond, nanosecond, and microsecond timescales
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. A further note of importance is that for the CPMG settings used, R^sub 2^(δ^sub 1^) is measured in the high ω^sub 1eff^ limit (ω^sub 1eff^ > p^sub A^Δω^sub I^), so that R^sub ex^ is proportional to B^sup 2^^sub o^ independent of whether the exchange is fast or slow to a skewed population (33,34)
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. The same figure also shows the S^sup 2^^sub f^ contours when an additional nanosecond-timescale internal motion is present with time constant τ^sub is^ = 2 ns and order parameter S^sup 2^^sub s^ = 0
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. The fitted rotational correlation time for native TTR using bound ANS was found to be 27 ns, and for native TTR with bound Bis-ANS 22 ns
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.Physicochemical background for the fluorescence properties of DCVJ and ThTTo understand the physicochemical background for the fluorescence of DCVJ and ThT when bound to misfolded TTR aggregates, we used glycerol-water mixtures to decrease the intrinsic rotational relaxation time of the fluorescent dyes
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. This approach has been employed here to deconvolute the fibrillar structure shown in Fig
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