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. Since ACYI can maintain its dimeric structure at a high GdnHCl concentration of 2 M (18), it seems that the dimerization domain was quite stable against denaturation
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. Hence, one must be cautious in interpreting observations of nuclear Overhauser effects as evidence for residual, long-range structure in highly denatured states
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. Conversely, distance distributions for residues that are farther in sequence tend to be broad and peaked at large distances
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. At this pH the protein has a net negative charge, the dimer carrying a charge of -17e (36), where e is the protonic charge; and so the apparent specific volume would be expected to have a contribution from net repulsive electric double layer interactions in addition to the excluded volume of the hydrated protein itself
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. Second, the heat-unfolding curve of the apoA-2:DMPC disks is sigmoidal and is shifted to higher temperatures as compared to that of free protein; thus the unfolding of free apoA-2 is complete at 80°C, well below the apparent melting temperature T^sub m^ = 84°C of the apoA-2:DMPC complexes observed at this scan rate (Fig
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. When key stabilizing interactions succeed in reducing the dimensionality of the search space more drastically, much higher values can result (from differentiation of the higher-dimensional analogs of Eq
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. All specimens examined had 33-40 total myomeres (mode:36-38 myomeres)
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. The spleen cells obtained by this procedure were fused with the myeloma cell line X63-AG8
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.MORPHOLOGY OF TONER PARTICLESFigure 5 shows an SEM image of the Colorocs toner, revealing irregularly sharp-edged particles
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