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. All other reagents were of the highest analytical grade available
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. Spectra were collected from 30°C to 98°C at intervals of 2°C with a spectral resolution of 4 cm^sup -1^ and 256 scans
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. making the monomermonomer interaction less stable
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. The latter feature shows that weak binding sites, similar to those induced in azurin by the destabilizing salt KSCN (12), are common with proteins under these conditions
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. Since the diameter of this gorge, at its narrowest point, is smaller than the cross-section of the quaternary group of ACh, substantial breathing motions of the whole AChE catalytic subunit are necessary to allow the substrate to penetrate to the active site
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.Deriving the activation barriers assuming a two-state exchange is likely to be a significant simplification, as the conformational landscape may be much more complex
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. Equilibrium cluster formation in concentrated protein solutions and colloids
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. ANS bound to aggregated TTR showed that the minor component increased its lifetime to 4
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. The same is true for DCVJ (Fig
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