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Similar to open source, but with a focus on concept rather than coding, Browster created the community-based program to improve and enhance its beta version plug-in
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. Representative isothermal and thermal denaturation profiles show that glycosylation accounts for a stabilization of ~9 kcal/mol of the tetramer, whereas the difference in T^sub m^ between the two forms is 26°C
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. Regions shaded in black are contacts that are pronounced in the denatured state
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. coelicolor has been reported, and the corresponding proteins cloned and expressed (14-16)
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. In this work, between pH 6 and 8
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. Proteins of the phosphoenolpyruvate:sugar phosphotransferase system in Streptomyces: possible involvement in the regulation of antibiotic production
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. CFTR deficiency also alters the epithelial response to apoptotic cells, resulting in the enhanced release of the neutrophil chemoattractant IL-8
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. To remove the hydrophobic modifications, the protein was treated with 1mM dithiothreitol
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. This value of E^sub a^ is comparable to the activation energy for the slow phase of apoA-2:DMPC disk denaturation (Fig
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. Interestingly, in these two samples (1 h and 1 week incubation at 70°C) the fibers do not criss-cross
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. The reactivity of the antibodies produced by the hybridomas was then determined
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. The relatively narrow and symmetric peak observed for the WT 4WJ under the same conditions is overlaid for comparison
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. This agrees with the DSC data from an identical sample indicating that disk reconstitution after heat denaturation takes more than 48 h to complete at 24°C (Fig
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