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. Altering kinetic mechanism and enzyme stability by mutagenesis of the dimer interface of glutathione reductase
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. Despite the large amounts of ACYI that exist in mammalian kidney and liver, the reactivation of ACYI is particularly difficult in vitro (19-21), which suggests that the folding of ACYI in vivo might be assisted by the possible intracellular molecular chaperones and/or osmolytes
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. For the 28 proteins studied by Kohn et al
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. The N-terminal domain showed basically the same structure as the EIN^sup ec^ (8,9), since the alignments for homology modeling showed only minor deletions in loops between α-helix I and α-helix 2, α-helix 2 and α-helix 3, and α-helix 3 and α-helix 4 (Fig
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. Conformational stability of dimcric proteins: quantitative studies by equilibrium dcnaturation
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. William VandivierApoptosis and the removal of apoptotic cells (termed efferocytosis) are tightly coupled with the regulation of normal lung structure, both in the developing and adult organism
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. Three fluxes (Alpha) having the surface tensions of 370 mN/mm for type NC (no-clean), 445 mN/mm for type R (nonactivated), and 415 mN/mm for type WS (water soluble) were used to evaluate the wettability under different solder/flux surface tension conditions
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. Thus, activation by an agonist through the PLCβ-G-proteins pathway affects the activity of a host of Ca^sup 2+^-sensitive enzymes
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. The molecular details of how PLCβ interacts with Gβγ is unknown
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. 193 K/ min (from the apparent T^sub m^ = 84°C to 75°C)
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. Smaller posttransition juveniles (BL [less than or equal to] 33 mm) retained some of the larval pigmentation pattern
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. 1% Tween 20/PBS, and incubated with 4-chloro-1-naphthol solution (substrate of horseradish peroxidase in the kit) containing 0
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. At low Mg^sup 2+^, all three ribozymes show a single peak at low E, reflecting an extended, undocked state
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. Currently, we are unable to unambiguously rationalize the difference between the behavior of the immobilized 4WJ ribozyme and the 4WJ construct examined in our solution-based spFRET experiments
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. For comparison, the δ values calculated from native structures are also shown as open circles
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. Thermal denaturation measurements Experiments were performed with a response time of 8 s and at constant heating rates of 15, 30, and 60 K/h to test for microreversibility (6), in the presence of 1 mM β-mercaptoethanol
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.The aggregation reaction was initiated through a pulse of NaCl which induces the molten-globulelike A-state which subsequently assembles into aggregates
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. These aggregates have a size of ~500 kD, which results in an average concentration of oligomers of ~30 nM
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