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As a result, for each sequence, we generated an ensemble consisting of 10^sup 4^ uncorrelated conformations
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Characterization of cold denaturation and urea-protein interactions
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Structures of product and inhibitor complexes of Streptomyces griseus protease A at 1
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This work was supported by grants from The Nanotech Programme of the Swedish Defence Research Agency (M
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The pleckstrin homology domain of phospholipase C-β^sub 2^ links the binding of Gβγ to the catalytic core
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The first just gives the large dimensionality of hypersphere, the second the flat diffusive landscape
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As we have remarked above, however, candidates for such long range forces do not present themselves
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Trachinoidea: development and relationships
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The backbone atoms (N, Cα, and C') of the most stable structural elements of both proteins, the first α-helix of gp120 (residues 17-31), and part of the β-sheet of CD4 consisting of residues 26-30 and 82-86 (see Fig
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The target toner particle size for printing with the Colorocs printer is 5 to 20 µm
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These intrinsic features of RNA folding reactions can be obscured due to the averaging inherent in conventional ensemble experiments
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. All the significantly exchanging residues are clustered in well-defined regions, namely on the CaM: SEF2-1 mp interaction interface, in the CaM:CaM dimerization interface or in the flexible hinge region that connects the N- and C-terminal domains within the CaM monomer
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. Bound to aggregated TTR, the fluorescence lifetime of both components have increased and the relative amplitude of the slower component has increased (Table 3), well in accordance with the massive increase in quantum yield compared to when bound to native TTR (Fig
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. For typical membrane concentrations used in these studies, the apparent K(d) should be ~200-fold stronger than the true K(d) (see Runnels and Scarlata, 1999)
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. An example of the behavior of CPM-PLCβ at subsaturating (i
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. Such analysis nonetheless enables us in the current study to estimate the entropic contribution of subsets of atoms, or degrees of freedom of interest, down to residue and atomic levels
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. At 130°C, the mixtures had to be held for 2 hours for the polymer to appear to be completely dissolved
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.To confirm that our labeled ribozyme constructs are active in cleavage, we performed activity assays using a cleavable all-RNA substrate strand, as described in the Materials and Methods section
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