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The proteins were redissolved in D^sub 2^O and centrifuged at 6000 × g for 10 min before use with a final pD of 8
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Although phase diagrams have usually been used in the analysis of Trp fluorescence data in previous publications, the theoretical analysis of the phase diagram is also expected to be true for IR data
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coli, EIN^sup ec^ (9), and the C-terminal domain of T
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In the case of azurin, to be consistent with the previous study (12), the protein concentration was raised to 2
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Following this procedure, reproducible ( Fluorescence measurements Fluorescence measurements were conducted on a homemade fluorometer
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The preceding study (12) has demonstrated that the creation of hydrophobic ANS binding sites in ice, accounting for the spectral blue shift and intensity enhancement, is owed to partial disruption of the protein tertiary structure triggered by the solidification of water
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Diminazene peaks were not observed within 6 h, whereas aceturate peaks slowly grew with time
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However, at the relatively high CaM concentration (1 mM) used in the NMR experiments, compared to the nanomolar dissociation constant of the complex, all CaM is in the bound state
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Size distribution functions were computed using the appropriate Windows-based ALV software, which incorporated regularized inverse Laplace transform and ALV-CONTIN packages
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On solid-like rheological behaviors of globular protein solutions
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Structure and evolution of the apolipoprotein multigene family
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It is possible here to provide only a broad overview of the relationships involved
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7% in summer and fall surveys, respectively
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2] of sea surface) were found in December 1986, 1996, and 1999
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Since overlap between blocks can reduce the standard deviation and hence the standard error (Fig
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5 Spent Ovary flaccid, purplish- Early-stage oocytesred in color
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These findings strongly suggest that the method developed in this study is useful for identifying P
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Docking of the two loops is also observed in simpler ribozyme constructs comprised of two- or three-way helical junctions (2WJ and 3WJ, respectively), although much higher Mg^sup 2+^ concentrations are required (N
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