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. Instrumental aspects and data analysisNeutron scattering experiments were carried out on the backscattering spectrometer IN16 (energy resolution ΔE = 0
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. That a two-contribution model is needed follows from a comparison of average experimental and theoretical R^sub 1^ values
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. In addition ANS shows a minor slow second phase in response to formation of larger assemblies (spherical aggregates and protofibrils)
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. Taken together, these data indicate that heating of the apoA-2:DMPC disks to 99°C leads to protein unfolding and disk-to-vesicle fusion; consecutive cooling leads to a gradual reduction in the vesicle size and partial protein refolding, followed by formation of smaller protein-containing vesicles and additional helical refolding at 34-24°C
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. Another difference between the 10-min insulin aggregate preparations at 60 and 70°C concerns diversely convoluted shapes, whose presence is more pronounced in the latter case
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. It is generally accepted from thermodynamic and biochemical data that the β-sheet of gp120 that bridges the inner and outer domain of the gp120 core, the bridging sheet, is only fully formed and stabilized upon complexation with CD4 (Fig
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. 5-, 1-, and 2-ns block sizes) were used for fitting the standard errors to an exponential function using the xcrvfit software (http:// www
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. A conserved HIV gp120 glycoprotein structure involved in chemokine receptor binding
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. The fluorescence emission was collected using the same objective, separated from the excitation light using a dichroic filter (540 DRLP, Omega Optical, Brattleboro, VT), spatially filtered using a 100-µ pinhole, and separated into donor and acceptor components using a second dichroic filter (630 DRLP, Omega Optical)
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