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. The hydrodynamic radius of rSBA as obtained from dynamic light scattering studies is ~4
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. that the large amphipathic surface in the dimer interface could be exposed to solvent upon monomer formation; furthermore, there is a network of aspartic residues involved in salt bridges at the dimeric interface (Fig
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. J Exp Med 2001; 194:781-796(24) Vandivier RW, Ogden CA, Fadok VA, et al
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. In the case of multitryptophan proteins, tyrosine fluorescence emission is generally not visible, mainly due to much larger absorption coefficients and quantum yields of the tryptophans, and at least for folded states because of distinct Förster energy transfer from tyrosines to tryptophans (3,14)
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. Harmonic behavior of trehalose-coated carbon-monoxy-myoglobin at high temperature
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. The angle that a helix axis makes with the main axis of the diffusion tensor can therefore be determined with reasonable accuracy from (τ^sup o^^sub m^)^sub app^ averaged over a helix
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. Universal features of the fluid to solid transition for attractive colloidal particles
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. Physicochemical background for the fluorescence properties of DCVJ and ThTTo understand the physicochemical background for the fluorescence of DCVJ and ThT when bound to misfolded TTR aggregates, we used glycerol-water mixtures to decrease the intrinsic rotational relaxation time of the fluorescent dyes
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- "You can control your expenditures somewhat," says Rosenbloom, "but I know of nobody who can accurately predict their collections
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. The R^sub g^ of molten globule (MG) state is close to that of N state rather than U^sub A^ state
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. The co-founder of the theory of evolution by natural selection gave an explanation that was, in the end, remarkably Lamarckian
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. Details of these tests for convergence of the simulations and the sample size are presented in the Appendix
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. In C3A/C26A the elimination of the superficial C3-C26 disulfide bridge, linking the ends of strands β land β2, has little effect on the compactness of the protein core and, judging from the phosphorescence properties of W48 embedded in it (18), internal motions remain restrained
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. At high volume fractions there is a sufficient slowing of particle dynamics such that liquidlike configurations cannot be explored over practical timescales (5,6)
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. When both the amplitudes and the rates are taken into context, it is evident that ThT responds slowest of all probes in the study, which suggests that its strongest signal emanates from the most mature fibrils
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. This will be the more likely as secondary structure forms, as we shall see by the example of a three-helix bundle below
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