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. ANS binding studies ANS (1-anilino-8-naphthalene sulfonate) binding experiments were executed with both the native and recombinant protein
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. we plot ln([left angle bracket]R^sub ij^[right angle bracket]) versus ln(|j - i|) for four representative sequences drawn from Table 1
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. From the difference contact maps, we find that upon folding, specific nonnative local contacts have to be broken (weakened) to make native, nonlocal spatial contacts
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. Funnels, pathways, and the energy landscape of protein folding: a synthesis
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. And the trend may be increasing
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. In addition, it provides important structural information
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. This monomer-dimer equilibrium is dependent on ionic strength and protein concentration
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. Bis-ANS shows a wide variety of fluorescence responses and fluoresces most strongly when bound to monomeric A-state TTR and surprisingly also fluoresces strongly when bound to unfolded monomeric TTR
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. Another recent, theoretical, contribution has pointed out that fine-structure of a few k^sub B^T within the transition state on a reaction pathway can accelerate folding (Wagner and Kiefhaber, 1999)
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. [infinity] = asymptotic length; k -- Brody growth parameter; [t
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. Since we use relative peak positions and distance (inter-arm proximity) changes in our discussion here (and not absolute distances), variations of [gamma] from 1 do not affect the discussion or conclusions of this study
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- The last number, "Greystone Chapel," written by Glen Sherley, an inmate in the audience, thanks God for the only place at the prison whose door was never locked: Now this Greystone Chapel here at Folsom-- It has a touch of God's hand on every stone
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.Blindness in Astyanax seems to be caused instead by mutations in a small number of master switches that control other genes and proteins necessary to the development of the eye
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. Interestingly, the results from fluorescence and infrared (IR) spectroscopy clearly indicated that pretransitional conformational changes occurred at temperatures from 50°C to 66°C during ACYI thermal denaturation
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. Interestingly, the difference in T^sub m^ of the two forms is ~26°C, and this estimates the extent of stability imparted by the oligosaccharide chains to the stability of gSBA
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. and would alter the fluorescence of Trp461 (Fig
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.In this work, the wettability of newly developed Sn-2
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. A novel, specific interaction involving the Csk SH3 domain and its natural ligand
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. Therefore the conserved A6-A11 SS-bond is expected to cause a significant topological constraint and bending of the A-chain
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. But when there are many sequential subspaces, an apparently continuous folding funnel appears with all of the features of intermediate states, multiple pathways, etc
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. Ovary connective tissue was retained in the coarse sieve, and freed eggs were collected in the fine-mesh sieve
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.A more infamous example is the drug thalidomide which was widely and aggressively marketed in the fifties and sixties as "a harmless tranquillizer particularly suitable for the pregnant woman" since it has been shown to be not teratogenic "after repeated and rigorous animal tests (p
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