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. Using CPM-PLCβ as a readout of possible conformational changes corresponding to Gβγ activation, we monitored CPM-PLCβ fluorescence under pressure and compared it directly to using CPM-PLCβ with supersaturating levels of Gβγ-subunits
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. The C^sub p^(T) data were recorded from degassed protein and/or buffer solutions upon repetitive heating from 5 to 100°C at a rate 90 K/h, followed by cooling and incubation at 24°C
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. Another unanticipated result is the observation of a repeatable lipoprotein transition that is thermodynamically irreversible
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. By combining the trajectories of the free and complex forms, further insight into the conformational sampling was extracted
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. Assessment of the complete system was not yet feasible due to insufficient sampling, especially for gp120
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