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. The PTS involves a cascade of phosphoryl-transfer steps from phosphoenolpyruvate (PEP) via phosphointermediates of the general cytosolic non-sugar-specific phosphotransferases enzyme I (EI) and the histidine-phosphocarrier (HPr) to sugar-specific enzyme II permeases
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. Importantly, a very interesting correlation was evident from these measurements showing that the limiting anisotropy values correlates to the protein concentration, indicating that this is a possible method for detecting aggregation formation and aggregate size distribution
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. Iodide is a negatively charged quencher expected to quench the fluorescence emission of solvent-exposed tryptophan residues (Lakowicz, 1983)
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. 3, we tested internal motion models with either one (M1; τ^sub 1^ and S^sup 2^ fitting parameters) or two contributions (M2; τ^sub if^, S^sup 2^^sub f^, τ^sub is^ and S^sup 2^^sub s^ fitting parameters) to the internal motion
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. We treated Gα^sub s^(GDP) with dithiothreitol (see Philip and Scarlata, 2004) and found that its pressure behavior was unchanged (data not shown)
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