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It is tempting to suggest, based on our results, that the species populated at high pH is also a molten-globule-like species, although it did not bind ANS (Fig
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Thermodynamics and kinetics of unfolding of the thermostable trimeric adenylate kinase from the archeon Sulfolobus acidocaldarius
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(32) The first pathway includes UNC-73, MIG-2, ced-12, ced-5, and ced-2 (representing the mammalian homologues TRIO, RhoG, ELMO, Dock 180, and CrkII, respectively)
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The remaining residues (see Table 1) show a weak decrease in exchange rate upon change in temperature (f [asymptotically =] 0
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Time-resolved fluorescenceTime-resolved measurements of tryptophan (Trp) fluorescence were recorded using an IBH (Glasgow, UK) 5000 U fluorescence lifetime spectrometer system with 1 nm resolved excitation and emission monochromators (5000 M)
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Aggregates are expected to have rotational correlation times considerably longer than the decay time of the used dyes (e
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The data reveal that DCVJ is a preferable probe for detection of early oligomers given its early response in the kinetic assay although it also binds to more mature fibrils and spherical aggregates
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Maximum summer densities of posttransition pinguipedids were found southeast of Peninsula Valdes, whereas greatest fall densities were detected northeast of Camarones Bay (Fig
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