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Apart from its crucial functions in intracellular metabolisms, ACYI also has substantial commercial importance for the optical resolution of N-acylated DL-amino acids
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Generation of conformational ensembles We have adapted conventional Markov-chain Metropolis Monte Carlo simulation strategies (60,61) to generate equilibrium ensembles for each of the protein sequences in the EV limit
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Use of fluorescence decay times of 8-ANS-protein complexes to study the conformational transitions in proteins which unfold through the molten globule state
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A sample of ~200 mg DmAChE was dialyzed against, and then lyophilized from 0
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A novel target recognition revealed by calmodulin in complex with the basic helix-loop-helix transcription factor SEF2-1/E2-2
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The spectra obtained upon Trp excitation (290 nm) are shown in Fig
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The plateau level is preserved for DCVJ over 300 h, showing that this probe also binds to larger assemblies (spherical aggregates and protofibrils)
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Comparison of the configurational entropies of gp120 and the CD4 D1 domain (denoted CD4 for simplicity in the following) in the free and bound states suggests that the formation of intermolecular hydrogen bonds and hydrophobic contacts contributes most to the entropy changes
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The globules become visible when background stars lie near enough to illuminate them
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THERMAL ANALYSISWe determined the crystalline melting temperatures T^sub m^, of the IPP, EVA 9%, and EVA 19% polymers by DSC as 165, 83, and 97°C, respectively
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RNA folding: models and perspectives
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It is important to note, in addition to the alteration in the expression of phenotypic markers, that pellets cultured in DMEM/ITS+/TGF-[beta]1 were larger with greater amounts of DNA and GAG compared with pellets maintained in DMEM/10%FCS
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