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He advises business owners to, at minimum, use an interest-bearing money market account to park excess cash
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. Nevertheless, the 2D IR analysis also suggested that the pretransitional conformational changes involved the unfolding of the solvent-exposed β-sheet structures and aggregates appearing at a temperature at which the protein had not fully unfolded
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. 1, σ^sub ij^ is the hard-sphere contact distance (54), r^sub ij^ is the interatomic separation, and the dispersion parameters ε^sub ij^ are determined by static polarizability values for individual atoms (55,56)
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. This energy difference is used with the Metropolis criterion (61) to accept or reject the proposed move
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. This contrasts with the tighter association of apoA-2 than apoA-1 with mature HDL, suggesting different molecular determinants for stabilization of model discoidal and plasma spherical HDL
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. These distances are in agreement with the corresponding values obtained from the present spFRET experiments (see Results section above)
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.5 M, the GR dimer eluted at a volume corresponding to ~120 kDa
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. As a consequence the observed emission spectra of individual proteins (and of their corresponding conformational states) do not even vary with respect to λ^sub max^ but also can show different spectral shapes and widths (7,8)
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. It should however be noted that Bis-ANS still fluoresces very strongly in the presence of large aggregates (Figs
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. This same volume change is obtained for a peptide derived from Gβ which also activates PLCβ
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.To compare the activation energy E^sub a^ of disk denaturation for different proteins, we recorded T-jump CD data of apoA-1:DMPC disks (Fig
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.[FIGURE 6 OMITTED]DiscussionLiterature describing the early stages of species belonging to the family Pinguipedidae (formerly Mugiloididae) is scarce
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.Temporal patterns in parturitionFrom 1996 through 1998 we estimated relative larval production for four age groups: 6-8; 9-11; 12-14; and 15 years and older (Fig
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