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. The heat flow versus temperature profile of ACYI contained an asymmetric positive peak arising from an exothermic transition (Fig
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. The observed red shift is caused by the fact that tryptophan residues are replaced from the less-polar interior of the protein to solvent exposed regions upon unfolding
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. Incoherent inelastic neutron scattering experiments probing the vibrational density of states are scarce but seem to favor an increase of low-frequency, collective motions resulting in increased protein flexibility upon ligand binding (10)
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. To speed up these calculations, we take advantage of the short range of inverse power potentials (53)
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. 6, which shows that deviations from rodlike behavior occur for all sequence separations greater than a single residue
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. Analysis of the pH- and thermal-denaturation curves The fittings to Eqs
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. Molecular graphics were created with PyMOL (38)
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. As a final remark we point out the close association found between ANS binding capacity (relative area, Table 2) and the peak wavelength of the fluorescence spectrum, λ^sub max^, the latter shifting further to the blue as the binding capacity increases
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. For Bis-ANS, the fluorescence in the presence of unfolded TTR is significant
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.We are grateful to Per Persson for initial assistance with electron microscopy work and to Jeffery Kelly and David Wemmer for providing the pmmHα plasmid
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.The effect of pressure on lipid membranes has been well characterized
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.We find that membrane binding of PLCβ, which is a chimera of PLCβ^sub 2^ and PLCδ^sub 1^, is pressure independent as expected from the pressure behavior of wild-type PLCδ^sub 1^ (Rebecchi et al
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. The broad asymmetric peak shape observed for the SL 4WJ implies that extended undocked and quasi-docked states are in rapid conformational exchange, and our kinetic simulations indicate that the rate constant for formation of the quasi-docked state is in the range 10-41 × 10^sup 3^ s^sup -1^, whereas the corresponding reverse rate constant is in the range 3
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