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"Mulford stands by his analysis
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) In terms of weight, the A7 is the heavier of the two at 6
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Two months later, the surface fish with a transplanted cave-fish lens had a highly degenerated eye
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Each of the subunits is glycosylated at the Asn-75 position (Fig
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Proteins in high concentrations of denaturants, such as 8 M urea or 6 M GdnCl, behave like chains in good solvents (3)
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Depending on the choice in step 3, the backbone φ,ψ, or side-chain torsions are set to random values in the interval [- 180°, 180°]
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MATERIALS AND METHODS Materials Imidazole, the different salts and acids used in buffer preparation, sodium pyruvate, and NaCl were from Sigma (St
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Address reprint requests to Martin Weik, Institut de Biologie Structurale CEA-CNRS, 41 rue Jules Horowitz, F-38027 Grenoble Cedex 1, France
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The cross sectioned samples were then finally gold coated and prepared for analysis
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We find that pressure does not increase the tendency of CPM-PLCβ toward the fluorescence emission properties of the activated, Gβγ-bound form (Fig
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© 2005 by the Biophysical Society0006-3495/05/01/172/12 $2
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, 1992) and, more recently, for Prolatilus jugularis (Ve1ez et al
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MOLSCRIPT-a program to produce both detailed and schematic plots of protein structures
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This work was done by D
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The potential of a more flexible resin, which was not friable enough to be ground to the required particle size, was assessed by applying the resin with colorant by means of a solvent medium
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None of the eggs fixed just after collection were morphologically identifiable
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35 µg/ml; BD Biosciences, Oxford, UK)
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