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. commission reports, with several directed at reauthorizing the Magnuson-Stevens Act (MSA)
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. Address reprint requests to Avadhesha Surolia, National Institute of Immunology, Aruna Asaf Ali Marg, New, Delhi-110067, India
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. The latter is a model for a soft, Gaussian coil with large-scale, albeit uncorrelated
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. Henson; personal communication; March 2006)
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. Furthermore, since transcription levels will remain low until the midblastula stage when development speeds up, the cytoplasm is stuffed with high amounts of maternal RNA (40)
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. Interestingly, a lack of enhanced membrane binding was also observed for the nonspecific PLCβ-membrane association, suggesting that the enzyme packs efficiently with the membrane interface
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. INTRODUCTIONHigh-density lipoproteins (HDLs) are heterogeneous complexes of lipids and specific proteins (termed exchangeable apolipoproteins) that mediate cholesterol removal from peripheral tissues to the liver via the process termed reverse cholesterol transport (Fielding and Fielding, 1995)
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. , 2003a), show that the heat- and denaturant-induced transitions in discoidal lipoproteins are kinetically controlled reactions that involve protein unfolding, dissociation, and lipoprotein fusion
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. Although different distribution profiles of fibrillar features may still be explained in terms of kinetic effects (temperature may differently affect the kinetics at various stages of the assembly, effectively marginalizing certain sequential processes), the higher temperature may also have a more direct effect on amyloidogenesis, for instance by increasing the thermal energy of the interacting molecules and hence causing the alignment of protofilaments to become less accurate
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. It was moderate to long and extended to near the midpoint of the body, resulting in a relative preanal length of 0
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. It summed up the furious outcry from many industry leaders already staggered by federal plans to cut billions of dollars in Medicaid reimbursements to pharmacies over the next several years
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. --Pharmaceutical Sciences* CALIFORNIAUniversity of California, San Diego School of Pharmacy and Pharmaceutical Sciences M
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. Journal of Social Psychology, 142, 405-424
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. Experiments were carried out at room temperature in 30 mM HEPES, at pH 7
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. 1 B), and most of the changes in anisotropy only took place between 2 and 5 M GdnHCl (Fig
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. 8 b shows the distribution of the iwo types of interactions
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. Thermal denaturations following the ANS fluorescence did not show a native baseline, and a sigmoidal-like decreasing of the fluorescence was observed as the temperature was raised (data not shown)
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. The dimerization surface included several salt-bridge networks, such as Asp^sup 467^-Arg^sup 424^', Arg^sup 456^-Asp^sup 358^', Asp^sup 540^-Arg^sup 422^', Asn^sup 345^-Asp^sup 448^', as well as backbone-backbone hydrogen bonds (Fig
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. Furthermore, in some cases the differences in intensity signals from folded and unfolded states are very small, which makes precise measurements difficult (12)
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. Structures of product and inhibitor complexes of Streptomyces griseus protease A at 1
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. Heat capacities and a snapshot of the energy landscape in protein GB1 from the pre-denaturation temperature dependence of backbone NH nanosecond fluctuations
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. The sample was thereafter stained using 2% uranyl acetate for 20 s, and the grids were dried overnight
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. This may possibly relate to the ability of the central part of apoA-1, but not apoA-2, to be displaced from HDL (Durbin and Jonas, 1997), with the N- and C-terminal segments of apoA-1 anchored in the lipoprotein and thereby precluding its fusion
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