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. Under reducing conditions, the number of SH groups in urea-denatured yeast GR increases to six SH groups/monomer (Massey and Williams, 1965), indicating full exposure of all the Cys residues in the protein
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. The quality of the final products was characterized to be homogeneous on size exclusion chromatography and polyacrylamide gel electrophoresis in the presence and absence of SDS, and the purity of the protein was estimated to be above 98%
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. whether the T^sub m^S are the same as at pH 7
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. The dependence of the ANS fluorescence intensity, in ice at - 13°C, on the starting ANS concentration in solution (10-200 µM) is shown in Fig
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. Having stressed that the data of Fig
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. Similar microscopic measurements and analysis were performed in solutions of PEG with a higher molecular weight and the same PEG concentration as used in the above experiments
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. Gα^sub i^(GDP) can then bind to activated PLCβ^sub 2^-Gβγ and perturb the weak activating interactions without affecting the strong binding interactions as depicted in the scheme shown in Fig
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. When the mutation of a residue gives rise to a value of Φ close to 1, it means that the change in folding rate arising from the mutation is consistent with comparable changes in the transition state and native state energies
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. The histogram shows two well-resolved (non-zero) peaks, centered at E ~ 0
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