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. 4, containing 150 mM sodium chloride (PBS) for 12 h at 4°C under constant stirring
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. In Flory's approach, a range of chain properties including R^sub g^, the average end-to-end distance (R^sub e^), the hydrodynamic radius (R^sub h^), the second virial coefficient (B^sub 2^), and the osmotic pressure (II) are calculated as series expansions in terms of the parameter z = N (T - Θ/T) (39,40,45)
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. Besides the shift of transition temperatures caused by different heating rates, pronounced differences in the λ^sub max^-values for the unfolded states are visible in Fig
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. To assure the absence of (salt or water) crystalline structures in the samples, we used the IN16 option to record a diffraction pattern in parallel to the elastic spectra (43)
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. , limited to the active site), concern low-frequency collective modes below the sensitivity of the technique used and/or present only on a longer timescale (> 1 ns), and not directly related to fast thermal fluctuations
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. Previous studies of the pressure behavior of PLCδ showed that pressure increased the reaction rate of substrate hydrolysis of this enzyme (Rebecchi et al
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. After the buffer baseline subtraction, the data were normalized to protein concentration
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. Demographic characteristics and descriptive statistics for the study sample are presented in Table1
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