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The mean residue ellipticity of the recombinant protein was similar to that of the wild-type, suggesting that there are no major structural changes between the native glycosylated and recombinant nonglycosylated proteins (Fig
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The PK^sub a^, leading to the acidic species could be determined from the ANSbinding and intrinsic fluorescence measurements, suggesting that one aspartic and/or glutamic acid must be responsible for this transition (43)
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Pervasive conformational fluctuations on microsecond time scales in fibronectin type III domain
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DISCUSSIONSufficient conformational sampling is a prerequisite for a reliable estimation of the conformational entropy
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Subsequent crystallization may occur to generate morphologies from these solutions
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Ligand-induced conformational changes observed in single RNA molecules
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