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. 4 shows quenching of GR fluorescence by iodide
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. Recently, the crystal structure of a D-aminoacylase from Alcaligenes faecalis was determined (16)
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. Direct demonstration of structural similarity between native and denatured eglin C
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. The thermal stability of EI^sup SC^, in the absence of PEP and Mg^sup 2+^, was maximal at pH 7
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. Structure of EI^sup SC^ by FTIR (steady-state measurements) In Fig
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. 1 A) and the maximum wavelength of the emission spectra moved toward higher values (data not shown), indicating solvent exposure of the fluorescence residues
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. Am J Respir Crit Care Med 2005; 172:972-979(53) Giles KM, Ross K, Rossi AG, et al
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. A possible explanation for the above correlation is that the drastic drop in water activity on ice formation causes the cavities to dehydrate and collapse with consequent rearrangement of the internal peptide structure
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. Detection of nano-second time scale internal motion and determination of overall tumbling times independent of the time scale of internal motion in proteins from ^sup 15^N relaxation data
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. Oxford Science Publications, Oxford
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. Time resolved fluorescence anisotropy of extrinsic probes as a measure of protein aggregationFluorescence lifetime data from ANS, Bis-ANS, DCVJ, and ThT were recorded from the probes bound to both native and aggregated TTR (Fig
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. These three situations, denoted cases A, B, and C, will be used in Results, below
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. The loss of entropy stemming from the rigidification of the residues in gp120 forming stable hydrophobic contacts to CD4-F43 (ΔS^sup 2nd,b-f^^sub gp120,F43cav^ = -0
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. Most of the residues of CD4 and gp120 that are involved in the intermolecular hydrogen-bond network (solid bars in Fig
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. Free energy determination of polypeptide conformations generated by molecular dynamics
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. Connect Tissue Res 1982:9:247-8
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.Circular dichroismFar-UV CD spectra of GR were measured on a JASCO (Tokyo, Japan) J-715 spectropolarimeter (kindly made available by Dr
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. In general, the thermal denaturation of proteins can be demonstrated by a two-state thermodynamic mechanism
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. Experiments were recorded between 300 and 400 nm
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. All the thermal and the calorimetric transitions were reversible and they could be fitted to a two-state model (Table 3)
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