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The culture of a thousand years is shattered with the clanging of the cell door behind you
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Then she turned professional and landed a large wedding for two hundred guests
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"But these improvements are a long way from the systemic changes that many critics believe are needed
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In a famous example, both St
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But they all have been reckoning that ''this has been greatlyoverdone,'' he said
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Transient association of newly synthesized unfolded proteins with the heat-shock GroEL protein
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Coming to the nature of the interactions, we see that both hydrogen bonding and hydrophobic interactions prevail in the system, the frequency of the latter occurring more often
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In high concentrations of denaturants, proteins behave like polymers in a good solvent and ensembles for denatured proteins can be modeled by ignoring all interactions except excluded volume (EV) effects
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For example, Fourier transform infrared spectroscopy experiments when conducted on freeze labile proteins have not detected significant changes in their secondary structure (2) presumably because they are carried out at relatively large protein concentrations, a condition in which the perturbation is often attenuated as if the protein itself acted as a stabilizer
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Lately, a study by Trp phosphorescence did provide direct evidence that the solidification of water causes important alterations of the native globular fold in all protein examined (3)
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8) is structurally and catalytically very similar to AChE (21)
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Sequence-specific binding of counterions to B-DNA
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The fluorescent dyes 1-anilinonaphthalene-8-sulfonate (ANS), 4-4-bis-1-phenylamino-8-naphthalene sulfonate (Bis-ANS), 4-(dicyanovinyl)-julolidine (DCVJ), and thioflavin T (ThT) were employed in both static and kinetic assays to characterize these oligomeric and protofibrillar states using both steady-state and time-resolved fluorescence techniques
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These aggregates have a size of ~500 kD, which results in an average concentration of oligomers of ~30 nM
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Color can deteriorate as well
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, 2000), they are the last pre-amyloidal state with a native secondary fold
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, neurotoxic protofibrils of α-synuclein-Rochet et al
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This can be avoided by ensuring the compatibility of the package with the product, minimizing exposure of package and product to offending odors and environments, and by ensuring that packaging is hermetically sound
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Influence of helical junction geometry on docking efficiencyWe obtained FRET efficiency histograms for three ribozyme constructs with different helical junction geometries (natural 4WJ as well as corresponding 2WJ and 3WJ constructs; Fig
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Our review of the literature on raptor pellets and our discussions with colleagues revealed no previous instances of an item as large as the primary feather being discovered incorporated into the body of a regurgitated pellet
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Another scientific method highlighted is computer simulation since the computers' "mnemonic ability" preempts "useless repetition of research" and facilitates their use as "experimental models" by feeding them with all "confirmed information" relevant to, say, human respiration
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The World Poker Tour, another set of tournaments located in casinos around the country, got picked up by the Travel Channel last year
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Nearly a decade ago, Congress funded the National Gambling Impact Study Commission to determine the economic and social consequences of legal gambling, and in 1999, after two years of study, the commission concluded that it was time "to consider a pause in the expansion of gambling
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One of the two powers with the ability to emerge as a rival-China-depends vitally on the Persian Gulf and Central Asia for its future supply of oil; the other-Russia-is heavily engaged in Iran, Central Asia, and the Caucasus region
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- Stabilization of proteins by glycosylation examined by NMR analysis of a fucosylated proteinase inhibitor
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. Folding and association of oligomeric and multimeric proteins
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. On freezing of the solution, however, the protein sharply enhances the probe fluorescence and shifts its spectrum to the blue
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. Heat capacities and a snapshot of the energy landscape in protein GB1 from the pre-denaturation temperature dependence of backbone NH nanosecond fluctuations
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.80, and τ^sup o^^sub m^ = 10 ns, an R^sub 1^ value of 1
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. Therefore, a purely diffusion-controlled on-rate k^sub on^ of 10 s^sup -1^ M^sup -1^ would put a lower limit on the CaM:SEF2-1mp affinity, k^sub off^/k^sub on^ > 10^sup -6^
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. NMR solution structure of a calmodulin-target peptide complex by multidimensional NMR
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. The aggregation reaction of monomeric acid unfolded TTR (14 µM) was initiated by addition of 25 µl 4 M NaCl to a 1000 µl sample, giving a final NaCl concentration of 100 mM (23°C)
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.De Cabo (3) described some osteological, meristic, and morphological characteristics of Argentine Sea pinguipedid larvae
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. 4a), yet minimal staining observed in the pellets cultured in DMEM/ITS+/TGF-[beta]1 (Fig
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. The mean physical component (PCS 12) HRQOL score for the entire sample was 51
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