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. One of the best-documented cases of pleiotropy is the gene associated with sickle-cell disease
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. The thermal dependence of ACYI activity was measured by incubating the enzyme solutions at given temperatures for 30 min, and then the activity assay was performed by mixing the enzyme solutions and reaction buffers preheated at the same temperatures
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. Hence, we can conclude that the intermediate obtained in the chaotrope-induced denaturation of rSBA is also monomeric in nature
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. The intermediate and high q regimes provide the most information regarding average chain shape and fluctuations (62-64)
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. For small x, the discrepancy is even more pronounced
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. Sugar transport by the bacterial phosphotransferase system
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. The raw data were corrected for pre- and posttransition slopes
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. Thermal unfolding transitions were monitored by taking the CD signal at 222 nm as a function of temperature
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. The pronounced blue shift for native proteins in NDSB solutions is most probably related to the fact that NDSB interacts with the surface of the proteins which causes a more hydrophobic nonpolar environment of surface exposed tryptophan residues
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. Briefly, we find: i), the observed 2
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. Purified proteins were dissolved in 8 M Urea and refolded by dialysis as described in Gursky and Atkinson (1996)
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. Samples were fixed in a solution of 5% formalin to seawater
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. Even though the abundance and distribution data used in our study came from cruises that targeted other species, they provide satisfactory spatiotemporal coverage
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. Such analysis nonetheless enables us in the current study to estimate the entropic contribution of subsets of atoms, or degrees of freedom of interest, down to residue and atomic levels
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. Hence, the 4WJ ribozyme is unique in its ability to fold into a structure with arms A and B in close proximity, similar to the native ribozyme, even in the absence of specific loop-loop docking interactions
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. In a 1941 Gallup poll, 24 percent of those surveyed said they regularly played; the total amounts wagered were so substantial that throughout the 1940s, '50s, and early '60s, politicians routinely floated the idea of legalizing bingo or reviving federal and state-sponsored lotteries in order to get a cut of the action
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. The yield of the protein was 5-8 mg per liter of culture
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.1 is similar but is higher at the lower ionic strength
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. The Θ^sub 222^(t) data show slow unfolding on a timescale of 10^sup 2^-10^sup 3^ s (as compared to 10^sup -8^-10^sup -10^ s for the helix-to-coil transition in solution; Yang and Gruebele, 2003), implicating high free-energy barrier ΔG*
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