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. This led us to shift the excitation to a lower wavelength (270 nm) to minimize the contribution from Rayleigh scattering in the emission spectra
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. Essentially the same levels of sequence similarity and identity are obtained in the comparison between E
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. The preceding discussion suggests that harshly denaturing (as opposed to mildly denaturing) conditions can be thought of as close mimics of "perfect", rather than just good, solvents (50)
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