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William Jefferson's freezer, he became the rightful target of a storm of bipartisan angerWhen an FBI bribery investigation turned up $90,000 in cash stashed in Rep
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. Iodide is a negatively charged quencher expected to quench the fluorescence emission of solvent-exposed tryptophan residues (Lakowicz, 1983)
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. This two-stage scheme is similar to that from the chemical unfolding/ refolding studies of ACYI (18,19), which also indicated that there existed a dimeric, aggregation-prone folding intermediate
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. Here, [left angle bracket]R^sup 2^^sub e^[right angle bracket] is the mean-squared end-to-end distance, R^sup 2^^sub ij^ = |r^sub i^ - r^sub j^|^sup 2^, and we choose r^sub i^ and r^sub j^ to be the position vectors of α-carbon atoms of residues i and j, respectively
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. A similar principle underlies the design of energy landscape theories for folding kinetics that are based on Go models (105-107)
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. On a molar basis, the greatest apparent affinity and the largest values of H are exhibited by the tetramers LDH, YADH, and ALD, the smallest by the monomers azurin, β-gal, and α-amylase
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. Anilinonaphtalene sulfonate as a probe of membrane composition and function
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. IENS is, therefore, well suited for experimental investigations of thermal fluctuations within biological macromolecules under different biochemical conditions on timescales that match those of MD theoretical calculations
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. Consequently, the on-rate of the equilibrium between free and CaM-bound peptide will be faster than diffusion controlled, which explains the fast exchange and the high affinity observed in the CaM:SEF2-1mp interaction (11)
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. Experimentally these states have been principally observed in colloidal systems although it has been shown that there are strong analogies between the behavior of colloidal and concentrated globular protein solutions (2)
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. Viscoelastic and SAXS studies of the structural transition in concentrated aqueous colloids of ovalbumin and serum albumins
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. These include activation or inhibition of plasma enzymes that are essential in reverse cholesterol transport (Forte et al
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. After the buffer baseline subtraction, the data were normalized to protein concentration and expressed as molar residue duplicity, [Θ], No changes in [Θ] were detected with changes in the protein concentration in the range explored (5-600 µg/ml)
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. This suggests different modes of lipid-protein interactions in plasma spherical and model discoidal HDL, which may result from different chemical composition and/or geometry of these particles
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. INTRODUCTIONThe growing body of evidence linking Alzheimer, Parkinson, or Creutzfeldt-Jakob diseases to protein misfolding and subsequent aggregation into the so-called amyloids (Harper et al
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. , 2004b), lag phase is a typical feature of protein amyloidogenesis corresponding to the thermodynamically unfavorable nucleation process (Bouchard et al
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. Chemokine receptors as HIV-1 co-receptors: roles in viral entry, tropism, and disease
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. In his 1983 study, MIT economist Peter Temin found that doctor visits for the common cold fell by 110,000 a year between 1976 and 1989 as the FDA switched cough and cold medicines to over-the-counter status
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