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. Size exclusion chromatography was used to investigate the quaternary structure of GR (Fig
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. Classical properties of the MG state include increased molecular volume caused by an increase in internal water content (Kuwajima, 1989), preservation of nativelike secondary structure (Nozaka et al
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. Dynamic light-scattering studies The dynamic light scattering measurements were done using a DynaPro-MS800 dynamic light scattering instrument (Protein Solutions, Lakewood, NJ)
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. The glycan was interacting with the protein via 27 hydrogen bonds and 53 hydrophobic interactions (Fig
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. The Saccharomyces cerevisiae nucleoporin Nup2p is a natively unfolded protein
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. Due to the complexity of the investigated proteins (multitryptophan proteins in an ensemble of structurally heterogeneous unfolded states) a quantitative relation between λ^sub max^-values and the extent of aggregation or the compactness of the unfolded state cannot be given
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. Dynamic regimes and correlated structural dynamics in native and denatured α-lactalbumin
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. Competitive electrostatic binding of charged ligands to polyelectrolytes: planar and cylindrical geometries
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. Time-resolved fluorescenceTime-resolved measurements of tryptophan (Trp) fluorescence were recorded using an IBH (Glasgow, UK) 5000 U fluorescence lifetime spectrometer system with 1 nm resolved excitation and emission monochromators (5000 M)
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. An interesting aspect of the late stages of the amyloidogenic assembly is provided by AFM data on insulin samples heated at 70°C for 20 (Fig
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. The pigmentation on the ventral body surface, between the isthmus and the anus, consisted of small stellate melanophores
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. Because of that, its intensity diminishes during storage at a rate dependent on storage temperature and the initial intensity of the cooked character
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. , at the lowest Mg^sup 2+^ concentration
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. 9 B, we plot a simulated histogram (bold line) using an equilibrium constant of 3 (in favor of the quasi-docked conformation), and forward and reverse rate constants of 20 × 10^sup 3^ and 6
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. Extended and docked conformations of the hairpin ribozyme were also resolved and quantified in previous ensemble trFRET experiments (N
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. Yet a cursory look at the lists of drugs still requiring prescriptions and those available over the counter belies the notion that drugs are so classified based purely on safety concerns
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. "The mission of our MFA design program is the designer as author and entrepreneur
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. "'You know the difference between me and you, Mr
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. Both Russian and Chinese oil companies had enormous development and supply contracts with Baghdad under Saddam Hussein, deals that are worthless in an Iraq controlled by the United States
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. "The dirty little secret is that even with really good science, there is still a lot of uncertainty about the stocks
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.Developing Asian countries had $497 billion in reserves at theend of 1997
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. As can be seen in Fig
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. The spectra were collected at a scan speed of 50 nm min^sup -1^
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. Analysis of the EV-limit ensembles suggests that the observations of local sequence-specific contacts are not incompatible with the observed power law behavior
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. Statistical Mechanics of Chain Molecules
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. Quenching experiments In experiments aimed to detect protein self-association, the concentration of EI^sup sc^ was varied at pH 7 from 0
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. De Matteis, and A
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. Although its physiological role is still unknown (22), it has found use as a stoichiometric bioscavenger for toxic organophosphate nerve agents (23)
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. However, these binding modes are essentially different from those of CaM-SEF2-lmp, because the target-binding proteins do not undergo large-scale motion in the bound state
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. of Physical Chemistry-Laboratory of Biophysical Chemistry, University of Nijmegen, Toernooiveld 1, 6525 ED, Nijmegen, The Netherlands
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. High pressure dissociated PLCβ-Gβγ with a ΔV = 34 ± 5 ml/mol
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. On the other hand, seeding experiments suggest that mature, solid fibrils act as the most effective catalysts of amyloidogenesis in vitro (Harper et al
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