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. The dielectric spectra of protein aqueous solution have usually manifest at least three relaxation processes called delta, and gamma (Grant et al
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. The attempts to avoid artifacts in the 2D IR plots have been described in detail elsewhere (30)
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. This article reports isothermal and thermal denaturation studies on gSBA and rSBA
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. Finally, the ensemble-averaged topology should be invariant with sequence or chain length and the chain is best described as a fractal object of dimension 1
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. The ensemble-averaged form factor, i
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. 3 µs and a repetition time of 2
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. To examine the reason for this factor, we compared T^sub 2^, the spin-spin relaxation times, of the methylene peak at 1
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. Thus, CaM can, in a Ca^sup 2+^-dependent manner, directly interact with some members of the bHLH family
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. It was shown in a seminal article by Semisotnov and co-workers that a blue shifted ANS fluorescence spectrum and a strongly increased quantum yield is diagnostic of binding to a molten-globule-like state with exposed hydrophobic patches (Semisotnov et al
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. The fluorescence lifetime of ANS was found to be the longest and therefore the most reliable in trying to resolve rotational correlation times of TTR oligomers and larger assemblies
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. Although we predict similar volume changes for this association in the two different environments, the apparent affinities will differ because the effective concentration of membrane-bound proteins tends to be much higher due to their confinement on the reduced area of the membrane surface giving stronger apparent affinities (see Runnels and Scarlata, 1999)
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. The formation of the globule itself from the denatured state corresponds in this picture to the first hypergutter in a series
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