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. , 1997), suggesting a high degree of intersubunit cooperativity in the reactivation of the enzyme
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. Two-dimensional infrared correlation (2D IR) analysis was carried out using SDIAPP software developed in-house (27) according to the generalized two-dimensional (2D) correlation algorithm (29)
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. As shown in Fig
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. A reinvestigation by high resolution 1H NMR spectroscopy
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. Contrary to interpretations of many such experiments, numerous molecular simulations (7,23,99) and recent single-molecule experiments (75) provide little evidence of long-range residual structure under harshly denaturing conditions
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. For small x, the discrepancy is even more pronounced
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. Converse to observations in other EI proteins by DSC (6) and CD (52)
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. Pyruvate site of pyruvate phosphate dikinase: crystal structure of the enzyme-phosphonopyruvate complex, and mutant analysis
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. The role of dynamics in enzyme activity
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. Though the increase in immersion depth causes a little decrease in wetting force, the effect is noticeable for the meniscus height, as shown in Fig
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. In fact, the pattern of (τ^sup o^^sub m^)^sub app^ back calculated from the CaM:SMLCK complex when the diffusion tensor would be oriented along an axis perpendicular to the real diffusion tensor closely matches the pattern of (τ^sup o^^sub m^)^sub app^ found for the dimeric CaM:SEF2-1mp complex, Fig
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. Furthermore, the amplitude of the light-scattering changes in apoA-1:DMPC complexes is significantly smaller than that in apoA-2 complexes, suggesting that apoA-1 inhibits disk fusion more effectively than apoA-2
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. The resolution of image acquisition was 512 pixels per line (512 × 512 pixels/image)
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. Generalized two-dimensional correlation method applications to infrared, Raman, and other types of spectroscopy
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